Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 10 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS). As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer's disease (AD). Additionally, RTN4R may play a role in regulating the function of the gap junctions.
Product Name:
Mouse Nogo Receptor/NgR Recombinant Protein (RPES4712)
Product Code:
RPES4712
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
NgR,NgR1,NOGOR,Rtn4r
Accession:
NP_075358.1
Sequence:
Met 1-Ser 447
Fusion tag:
C-His-Fc
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized recombinant Mouse RTN4R Fc Chimera at 2 µg/ml (100 ?l/well) can bind biotinylated human RTN4 (GST Tag) with a linear range of 0.16-2.5 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the the mature form of mouse RTN4R (NP_075358.1) (Met 1-Ser 447) was fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 85 % as determined by SDS-PAGE
Mol Mass:
73.8 kDa
AP Mol Mass:
95-100 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
