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Recombinant Mouse Macrophage migration inhibitory factor/MIF Protein (RPCB1105)

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SKU:
RPCB1105
Size:
10µg
Tag:
NO-Tag
Reactivity:
Mouse
Expression Host:
E. coli
€129

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Recombinant Mouse Macrophage migration inhibitory factor/MIF Protein
SKU:RPCB1105
Size:10µg
Tag:NO-Tag
Reactivity:Mouse
Expression Host:E. coli
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Mouse Macrophage migration inhibitory factor/MIF Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:< 0.1 EU/μg
Purity:> 95% by SDS-PAGE.
Formulation:Lyophilized from a 0.22 μm filtered solution of 50mM Tris 150mM NaCl pH8.0.
Gene ID:17319

MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4 - 7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity (12).

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:P34884
Product ImageRecombinant Mouse Macrophage migration inhibitory factor/MIF Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 10-15 kDa.

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