Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI and LXN, is a cytoplasm protein which belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite of the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. In addition, Latexin is also implicated in Alzheimer's disease.
Product Name:
Mouse Latexin/LXN Recombinant Protein (RPES5004)
Product Code:
RPES5004
Size:
10µg
Species:
Mouse
Expressed Host:
E.coli
Synonyms:
MGC144352, MGC144353
Accession:
NP_058033.2
Sequence:
Glu 2-Glu 222
Fusion tag:
N-His
Activity:
Measured by its ability to inhibit carboxypeptidase-A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis(2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al, 1999, J. Biol. Chem. 274:30468). The IC50 value is <2.0 nM .
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mouse LXN (NP_058033.2) (Glu 2-Glu 222) was expressed, with a polyhistide tag at the N-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
26.3 kDa
AP Mol Mass:
32 kDa
Formulation:
Lyophilized from sterile PBS, pH 8.0, 10% glycerol
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
