Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.
Product Name:
Mouse IL13RA2/CD213A2 Recombinant Protein (RPES4513)
Product Code:
RPES4513
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
CD213a2
Accession:
NP_032382.1
Sequence:
Met 1-Lys 334
Fusion tag:
C-His-Fc
Activity:
1. Immobilized human IL13 at 20 µg/ml (100 ?l/well) can bind mouse IL13RA2 with a linear ranger of 0.5-2 µg/ml.2. Measured by its ability to inhibit IL13-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Lys 334) of mouse IL13R?2 (NP_032382.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 85 % as determined by SDS-PAGE
Mol Mass:
64.5 kDa
AP Mol Mass:
75-80 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
