Interleukin 5 Receptor alpha (IL-5 R?), also known as CD125, is a hematopoietin receptor that plays a dominant role in eosinophil biology. Mature mouse IL-5 R? consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain. The high affinity receptor for IL-5 is a complex that consists of the ligand binding IL-5 R? and the transmembrane common β chain (βc/CD131) which is shared with the receptor complexes for IL-3 and GM-CSF. IL-5 R? binds IL-5 at low affinity and then associates with preformed βc oligomers to form the signaling-competent receptor complex. IL-5 stimulation of CD34+ hematopoietic progenitor cells induces the up-regulation of transmembrane IL-5 R? followed by eosinophilic differentiation and activation. IL-5 R? also promotes the differentiation of basophils and B cells. Exposure of mature eosinophils to IL-5 attenuates their IL-5 responsiveness by inducing the down-regulation of surface IL-5 R? and increased production of soluble IL-5 R?.
Product Name:
Mouse IL-5RA/IL-5 R Alpha Recombinant Protein (RPES2004)
Immobilized Human IL-15RA&IL-15-Fc(Cat: PKSH032567) at 10µg/ml(100 ?l/well) can bind Human IL-5RA-His.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Interleukin-5 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Asp18-His339 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
37.6 kDa
AP Mol Mass:
48kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
