Recombinant Mouse IL-5 Protein (RPCB1699)

Product Name:	Recombinant Mouse IL-5 Protein
SKU:	RPCB1699
Size:	10µg
Tag:	C-6His
Reactivity:	Mouse
Expression Host:	HEK293 cells

Protein Description:	High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-5 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:	<0.1EU/μg
Purity:	> 97% by SDS-PAGE.
Formulation:	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:	16191

Interleukin 5 (IL-5) is a member of the interleukin family with a length of 115 amino acids. Interleukins are a group of cytokines (secreted proteins/signaling molecules) that were first seen to be expressed by white blood cells (leukocytes) and has been found in a wide variety of body cells. Interleukin 5 or IL-5 is produced by T helper-2 cells and mast cells. It helps to stimulate B cell growth and increase immunoglobulin secretion and is considered a key mediator in eosinophil activation. Interleukin 5 (IL-5) has long been associated with several allergic diseases, including allergic rhinitis and asthma. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. IL-5 also had something with the terminally differentiated granulocyte eosinophils. IL-5 was originally found as an eosinophil colony-stimulating factor. It has been proved to be a major regulator of eosinophil accumulation in tissues and can modulate eosinophil behavior at every stage from maturation to survival.

Storage:	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:	P04401

	Recombinant Mouse IL-5 Protein was resolved with SDS PAGE under reducing (R) and non-reducing (NR) conditions, showing single bands at 18-25 kDa and 36-50 kDa, respectively.
	Recombinant Mouse IL-5 stimulates cell proliferation of the TF-1 Mouse erythroleukemic cells. The ED₅₀ for this effect is 0.12-0.49ng/mL.