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Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein (RPCB0737)

SKU:
RPCB0737
Size:
10µg
Tag:
No tag
Reactivity:
Mouse
Expression Host:
E. coli
€209
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Description

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Product Name:Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein
SKU:RPCB0737
Size:10µg
Tag:No tag
Reactivity:Mouse
Expression Host:E. coli
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:< 1 EU/μg of the protein by LAL method.
Purity:> 95% by SDS-PAGE.
Formulation:Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:16181

Interleukin-1 receptor antagonist protein (Il1rn), also known as IL-1ra, IRAP or IL1 inhibitor, is a member of theinterleukin 1 cytokine family. This protein inhibits the activities of interleukin 1 alpha (IL1A) and interleukin 1beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. The mouseIl1rn gene encodes a 178 amino acids (aa) protein with a 26 aa signal peptide. Mouse Il1rn protein shares 26%and 19% identity with its homologues IL-1 beta and IL-1 alpha, respectively. Il1rn can Inhibits the activity ofinterleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP forsignaling, but has no interleukin-1 like activity. Recently, an recombinant human Il1rn protein is used in thetreatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:P25085
Product ImageRecombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 15-25 kDa.
Product ImageRecombinant Mouse IL-1Ra/IL-1F3/IL-1RN inhibit IL-1 alpha-dependent proliferation in D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 2.47-9.86 ng/mL in the presence of 3ng/mL of recombinant human IL-1 alpha, corresponding to a specific activity of 1.01×105~4.05×105 units/mg.

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