15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) , cranioosteoarthropathy (COA), and isolated congenital nail clubbing.
Product Name:
Mouse HPGD/15-PGDH Recombinant Protein (RPES1462)
Product Code:
RPES1462
Size:
20µg
Species:
Mouse
Expressed Host:
E.coli
Synonyms:
15-PGDH,AV026552
Accession:
Q8VCC1
Sequence:
Met 1-Ser 269
Fusion tag:
C-His
Activity:
Measured by its ability to bind Rhesus ErbB3-His in functional Elisa.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mouse HPGD (Q8VCC1) (Met 1-Ser 269) was expressed, with a polyhistide tag at the C-terusmin.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
30.6 kDa
AP Mol Mass:
30 kDa
Formulation:
Lyophilized from sterile PBS, pH 8.0, 20% glycerol
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
