ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Mouse HER3/ErbB3 Recombinant Protein (RPES3196)
Product Code:
RPES3196
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
C76256,Erbb-3,Erbb-3r,Erbb3,Erbb3r,Her3
Accession:
Q61526
Sequence:
Met 1-His 641
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.1. Immobilized mouse ErbB3-His at 10 µg/ml (100 ?l/well) can bind biotinylated human NRG1 (isoform Beta1) with a linear range of 0.018-0.125 µg/ml.2. Immobilized mouse ErbB3-His at 10 µg/ml (100 ?l/well) can bind biotinylated human NRG1 (aa 2-246)-Fc with a linear range of 6.25-100 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse ERBB3 (Q61526) extracellular domain (Met 1-His 641) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 93 % as determined by SDS-PAGE
Mol Mass:
70 kDa
AP Mol Mass:
95 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
