Recombinant Mouse FGF-9 Protein (RPCB1222)

Product Name:	Recombinant Mouse FGF-9 Protein
SKU:	RPCB1222
Size:	10µg
Tag:	No-tag
Reactivity:	Mouse

Protein Description:	High quality, high purity and low endotoxin recombinant Recombinant Mouse FGF-9 Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:	< 0.1 EU/μg of the protein by LAL method.
Purity:	> 90% by SDS-PAGE.
Formulation:	Supplied as a 0.22 μm filtered solution of solution of 20mM Tris-HCl, 250mM NaCl, 1mM EDTA, 20% Glycerol, 1mM DTT, pH 8.5
Gene ID:	14180

Fibroblast growth factor 9 is also known as FGF9, GAF, HBFG-9, SYNS3, and is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is also a mitogen for oligodendrocyte type 2 astrocyte progenitor cells, smooth muscle cells, pheochromocytoma PC12 cells, and BALB/3T3 fibroblasts. However, unlike FGF acidic and basic, FGF9 has no effect on human umbilical vein endothelial cells, and it has been demonstrated that FGF9 binds preferentially to the IIIc form of FGFR3. Although no typical signal sequence was found in FGF9, it is secreted efficiently after synthesis not in a conventional manner. In nervous system, FGF9 is produced mainly by neurons and may plays an important role in CNS development. FGF9 has been shown to interact with Fibroblast growth factor receptor 3.

Storage:	Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Reconstitution:	Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:	P54130

	Recombinant Mouse FGF-9 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kDa.
	Recombinant Mouse FGF-9 Protein promotes proliferation of BALB/3T3 mouse fibroblasts cells. The ED₅₀ for this effect is 2.52-10.06 ng/mL, corresponding to a specific activity of 9.94×104~3.97×105 units/mg.