Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.
Product Name:
Mouse EPO Receptor/EPOR Recombinant Protein (RPES5179)
Product Code:
RPES5179
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
Epor
Accession:
NP_034279.3
Sequence:
Met 1-Pro 249
Fusion tag:
C-His
Activity:
1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.1-0.5 µg/mL in the presence of 16 ng/mL Recombinant mouse EPO.2. Measured by its binding ability in a functional ELISA.3. Immobilized mouse EPOR-His at 10µg/mL (100?L/well) can bind biotinylated mouse EPO-His.The EC50 of biotinylated mouse EPO-His is 34.5-80.6ng/mL.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse EPOR (NP_034279.3) (Met 1-Pro 249) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
26.2 kDa
AP Mol Mass:
30-35 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
