Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
Product Name:
Mouse CPA2 Recombinant Protein (RPES1725)
Product Code:
RPES1725
Size:
20µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
CPA2,Carboxypeptidase A2
Accession:
Q504N0
Sequence:
Met 1-Tyr 417
Fusion tag:
C-His
Activity:
Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of DTNB.The specific activity is >4000 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse CPA2 (Q504N0) (Met 1-Tyr 417) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 92 % as determined by SDS-PAGE
Mol Mass:
46.6 kDa
AP Mol Mass:
47 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
