CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.
Product Name:
Mouse CD40LG/TNFSF5 Recombinant Protein (RPES2031)
Immobilized Human CD40-Fc(Cat: PKSM041225) at 2µg/ml(100 ?l/well) can bind Human CD40LG-His. The ED50 of Human CD40LG-His(Cat: PKSH033600)) is 13.84ng/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse CD40L is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
18.8 kDa
AP Mol Mass:
20 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
