Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.Immune CheckpointImmune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Mouse CD172a/SIRPA Recombinant Protein (RPES1242)
Product Code:
RPES1242
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AI835480,Bit,CD172a,P84,Ptpns1,SHP-1,SHPS-1,SIRP
Accession:
BAA20376.1
Sequence:
Met 1-Asn 373
Fusion tag:
C-Fc
Activity:
Measured by its binding ability in a functional ELISA.Immobilized rat CD47-His at 10 µg/ml (100 ?l/well) can bind mouse SIRPA-Fc, The EC50 of mouse SIRPA-Fc is 0.6-1.4 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse SIRPA (BAA20376.1) (Met 1-Asn 373) was fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
64.9 kDa
AP Mol Mass:
1001-20 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
