Low affinity immunoglobulin gamma Fc region receptor III (Fc gamma RIII/CD16) is a member of the Ig superfamily. Based on close relationships in their extracellular domains, the Fc gamma Rs have been divided into three classes composing of Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Mouse CD16 is a type I transmembrane protein having two extracellular Ig-like domains consisting of immunoglobulin domain, repeat, signa and transmembrane, transmembrane helix. It is expressed on a variety of myeloid and lymphoid cells and associates with Fc R gamma to deliver an activating signal upon ligand binding. Fcgr3 is IgG binding and activation or inhibition of immune responses such as antibody-dependent cellular cytotoxicity, phagocytosis, cell surface receptor signaling pathway and positive regulation of type I/IIa/III hypersensitivity.
Product Name:
Mouse CD16/FCGR3 Recombinant Protein (RPES0226)
Product Code:
RPES0226
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, Fc gamma Receptor III, CD-antigen 16, CD16, FcRIII, IgG Fc receptor III
Accession:
P08508-1
Sequence:
Ala31-Thr215
Fusion tag:
C-6His
Activity:
Immobilized Mouse IgG1 Fc(Cat: PKSM041044) at 10µg/ml(100 ?l/well) can bind Mouse CD16-His. The ED50 of Mouse CD16-His is 39.1ug/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Low affinity IgG Fc receptor III is produced by our Mammalian expression system and the target gene encoding Ala31-Thr215 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
22.0 kDa
AP Mol Mass:
35-40 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
