Fcgr4, also known as CD16-2, is one of the receptors for Fc region of IgG which involve in immune responses. Fcgr4 mainly functions in cellular response to lipopolysaccharide, NK T cell proliferation, regulation of sensory perception of pain, wound healing etc. Three groups are included for Fc ? receptors (FcR), and they are Fc ? RI (CD64), Fc ? RII (CD32), and Fc ? RIII (CD16). Among these, CD64 possess high affinity even for monomeric IgG, while CD32 and CD16 display a relative lower affinity for IgG. Genes encodes these receptors are diverse differing by species and cell types. The aggregation of FcR having immunoreceptor tyrosine-based activation motifs (ITAMs) activates sequentially src family tyrosine kinases and syk family tyrosine kinases that connect transduced signals to common activation pathways shared with other receptors. FcR with ITAMs elicit cell activation, endocytosis, and phagocytosis. Fcgr4 belongs to Fc ? RIII (CD16) group.
Product Name:
Mouse CD16-2/FCGR4 Recombinant Protein (RPES0592)
Product Code:
RPES0592
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Low Affinity Immunoglobulin Gamma Fc Region Receptor IV,FcgR4,4833442P21Rik,CD16-2,FcgammaRIV,Fcgr3a,FcgRIV,Fcrl3
Accession:
NP_653142.2
Sequence:
Gly21-Gln203
Fusion tag:
C-6His
Activity:
Immobilized Human IgG1-Fc(Cat: PKSH032558) at 2µg/ml(100 ?l/well) can bind Mouse FcgR4-His.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse Low Affinity Immunoglobulin Gamma Fc Region Receptor IV is produced by our Mammalian expression system and the target gene encoding Gly21-Gln203 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
21.9 kDa
AP Mol Mass:
28-35kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
