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Mouse Carboxypeptidase A1 Recombinant Protein (RPES2647)

SKU:
RPES2647
Product Type:
Recombinant Protein
Species:
Mouse
€1,179

Description

system_update_altDatasheet

Mouse Carboxypeptidase A1 Recombinant Protein

Carboxypeptidase A1 ?CPA1?is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of  residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.

Product Name:Mouse Carboxypeptidase A1 Recombinant Protein (RPES2647)
Product Code:RPES2647
Size:10µg
Species:Mouse
Expressed Host:HEK293 Cells
Synonyms:0910001L12Rik,Cpa
Accession:NP_079626.2
Sequence:Met 1-Tyr 419
Fusion tag:C-His
Activity:Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >6,000 pmoles/min/µg.
Endotoxin:<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:A DNA sequence encoding the mouse CPA1 (NP_079626.2) (Met 1-Tyr 419) precusor was expressed with a C-terminal polyhistidine tag.
Purity:> 96 % as determined by SDS-PAGE
Mol Mass:47 kDa
AP Mol Mass:42 kDa
Formulation:Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

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