Recombinant Mouse Autotaxin/E-NPP2 Protein (RPCB1652)
- SKU:
- RPCB1652
- Size:
- 10µg
- Tag:
- C-His
- Reactivity:
- Mouse
Description
| Product Name: | Recombinant Mouse Autotaxin/E-NPP2 Protein |
| SKU: | RPCB1652 |
| Size: | 10µg |
| Tag: | C-His |
| Reactivity: | Mouse |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Mouse Autotaxin/E-NPP2 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.1EU/μg of the protein by LAL method. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 20mM Tris，150mM NaCl，pH7.4 |
| Gene ID: | 18606 |
ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain . Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine . The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site . Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2 . ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis ( and to be up-regulated in several types of carcinomas including breast and lung .
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | Q9R1E6-1 |
 | Recombinant Mouse Autotaxin/E- NPP2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-140 kDa. |
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