X-ray repair cross-complementing protein 5 (86 kDa subunit of Ku antigen/ATP-dependent DNA helicase 2 subunit 2/ATP-dependent DNA helicase II 80 kDa subunit/CTC box-binding factor 85 kDa subunit/DNA repair protein XRCC5/Lupus Ku autoantigen protein p86/TLAA/XRCC5) is a nucleus and chromosome which belongs to the ku80 family. The XRCC5 gene encodes a single-stranded DNA-dependent ATP-dependent helicase. Chromosome translocation is associated with XRCC5. X-ray repair cross-complementing protein 6, also known as (5'-deoxyribose-5-phosphate lyase Ku70/ATP-dependent DNA helicase 2 subunit 1/ATP-dependent DNA helicase II 70 kDa subunit/70 kDa subunit of Ku antigen/ATP-dependent DNA helicase 2 subunit 1/CTC box-binding factor 75 kDa subunit/Lupus Ku autoantigen protein p70/Thyroid-lupus autoantigen/XRCC6) is a nucleus and chromosome which belongs to the ku70 family. Heterodimer of a XRCC6 and a XRCC5 subunit associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. Additionally, the dimer associates with NAA15, and this complex binds to the osteocalcin promoter and causes it to be expressed. XRCC5 & XRCC6 Heterodimer Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
XRCC5 & XRCC6 Heterodimer Recombinant Protein (RPES2837)
Product Code:
RPES2837
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
KARP-1,KARP1,KU80,Ku86,KUB2,NFIV
Accession:
P13010&P12956
Sequence:
Met 1-Ile732&Met 1-Asp609
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
