VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressors that regulate the activity of AMP-activated protein kinase family members, thus playing a role in various functions such as cell metabolism, cell polarization, apoptosis, and DNA damage response. Protein kinase domain-containing protein VRK1 is found in the nucleus. The VRK1 gene is abundantly expressed in human tissues and has a higher level of expression in actively dividing cells, such as those found in the testis, thymus, fetal liver, and carcinomas. VRK1 is a serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53, preventing the interaction between p53/TP53 and MDM2. PCH1, also known as pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease, is caused by a defect in VRK1. PCH1 is marked by an abnormally tiny cerebellum and brainstem; motor dysfunction beginning in the womb that affects both central and peripheral muscles; gliosis, which resembles infantile spinal muscular atrophy, and anterior horn cell degeneration. Human VRK1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human VRK1 Recombinant Protein (RPES1187)
Product Code:
RPES1187
Size:
50 µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
PCH1,PCH1A
Accession:
Q99986
Sequence:
Met 1-Lys 396
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human VRK1 (Q99986) (Met 1-Lys 396) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
