Vinculin is a focal adhesion and cytoskeletal protein that belongs to the Vinculin/alpha-Catenin family and is present in cells at cell-cell junctions and cell-extracellular matrix (ECM) adhesions. Vinculin is an Actin-binding protein and one of the components of the Actin-Linking Functional module, which detects and feels the extracellular environment's mechanical characteristics. Vinculin is also involved in the transfer, transduction, and regulation of mechanical force between the cytoskeleton and adhesion receptors. Vinculin has two structural states: an open (active) and a closed (inactive) form, which are controlled by conformational interactions between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival. Human VCL/Vinculin Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Vinculin is produced by our E.coli expression system and the target gene encoding Pro2-Gln1066 is expressed.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
117.0 kDa
AP Mol Mass:
115 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
