Protein ubiquitination is an important cell function for targeting aberrant or short-lived proteins for degradation. The three classes of enzymes that contribute to ubiquitination are ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. UBE2A encodes an E2 ubiquitin-conjugating enzyme. UBE2A enzyme activity is needed for post-replicative DNA damage repair and may be involved in transcriptional regulation. UBE2A is associated with cognitive disability due to alternative splicing. Because of various splicing, there are many transcript variants. UBE2A is associated with a variety of intellectual developmental disorders, X-linked syndromic, nascimento type, and Kaufman oculocerebrofacial syndrome. Protein metabolism and Class I MHC-mediated antigen processing and presentation are two of the UBE2A-interacting pathways. Human UBE2A Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human UBE2A Recombinant Protein (RPES1907)
Product Code:
RPES1907
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ubiquitin-Conjugating Enzyme E2 A, RAD6 Homolog A, HR6A, hHR6A, Ubiquitin Carrier Protein A, Ubiquitin-Protein Ligase A, UBE2A, RAD6A
Accession:
P49459
Sequence:
Met 1-Cys152
Fusion tag:
N-GST&6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Ubiquitin-Conjugating Enzyme E2 A is produced by our E.coli expression system and the target gene encoding Met1-Cys152 is expressed with a GST; 6His tag at the N-terminus.
Purity:
> 80 % as determined by reducing SDS-PAGE.
Mol Mass:
49.0 kDa
AP Mol Mass:
52 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 50mM HEPES; 150mM NaCl; 2mM DTT; 10% Glycerol; pH 7.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
