Transferrin receptor protein 1 (TFRC) belongs to the peptidase M28 family that is synthesized as a 172 amino acid (aa). TFRC regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. It binds one transferrin or HFE molecule per subunit and binds the HLA class II histocompatibility antigen, DR1. It Interacts with SH3BP3 and STEAP3, facilitates TFRC endocytosis in erythroid precursor cells. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. It positively regulates T and B cell proliferation through iron uptake.
Product Name:
Transferrin Receptor/TFRC Recombinant Protein (RPES4266)
Product Code:
RPES4266
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Transferrin receptor protein 1, TR, TfR, Trfr, T9, p90,CD71,T9,TFR,TFR,TRFR
Accession:
P02786
Sequence:
Leu101-Phe760
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human TFRC is produced by our Mammalian expression system and the target gene encoding Leu101-Phe760 is expressed with a 6His tag at the N-terminus.
Purity:
> 90% as determined by reducing SDS-PAGE.
Mol Mass:
74.9 kDa
AP Mol Mass:
80-90 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,5% Trehalose,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
