Recombinant Human TNFSF5/CD40 ligand/CD154 Protein (RPCB1236)
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- SKU:
- RPCB1236
- Size:
- 10µg
- Tag:
- N-6His
- Reactivity:
- Human
- Expression Host:
- HEK293 cells
Description
| Product Name: | Recombinant Human TNFSF5/CD40 ligand/CD154 Protein |
| SKU: | RPCB1236 |
| Size: | 10µg |
| Tag: | N-6His |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human TNFSF5/CD40 ligand/CD154 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1EU/μg |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 959 |
CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P29965 |
 | Active Recombinant Human CD40- L/CD40L/TNFSF5/TRAP/CD40LG Protein was determined by SDS- PAGE with Coomassie Blue, showing a band at 20-27 kDa. |
 | Immobilized Human CD40L at 5 μg/mL (100 μL/well) can bind Human CD40 with a linear range of 0.005-0.5 μg/mL. |
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