Recombinant Human TIM-3/HAVCR2/CD366 Protein (RPCB0318)
- SKU:
- RPCB0318
- Size:
- 10µg
- Tag:
- C-hFc&His
- Reactivity:
- Human
- Expression Host:
- HEK293 cells
Description
| Product Name: | Recombinant Human TIM-3/HAVCR2/CD366 Protein |
| SKU: | RPCB0318 |
| Size: | 10µg |
| Tag: | C-hFc&His |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human TIM-3/HAVCR2/CD366 Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | > 87% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
| Gene ID: | 84868 |
HAVCR2 also known as TIM3(T cell immunoglobulin and mucin domain-3), is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. An alternatively spliced isoform is truncated within the mucin-like stalk.TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity . TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA. TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | Q8TDQ0 |
 | Recombinant Human TIM-3/HAVCR2/CD366 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 60-80 kDa. |
 | Immobilized Recombinant Human Galectin9 at 2 μg/mL can bind recombinant human HAVCR2 with a linear range of 0.3-5 μg/mL. |
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