Transforming growth factor beta 3(TGFB3) is a member of a TGF -beta superfamily which is defined by their structural and functional similarities. TGF-beta3 is produced in a complex with LAP. TGF-beta 3 becomes activated after cleavage by plasmin, matrix metalloproteases (MMPs), thrombospondin -1, and a subset of integrins. It binds strongly to TGF-beta RII, a type II serine/threonine kinase receptor. TGFB3 is key in cell differentiation, embryogenesis, and development. It's been linked to the regulation of molecules involved in cell adhesion and ECM formation during palate development. Without TGF-beta 3, humans develop a cleft palate. Human TGFB3 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 mouse T cells. The ED50 for this effect is 10-80 pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human/Mouse Transforming Growth Factor beta 3 is produced by our Mammalian expressionsystem and the target gene encoding Ala301-Ser412(Tyr340Phe) is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
12.7 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 4 mM HCl.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
