Transforming Growth Factor beta-1 (TGF beta-1) is a secreted protein which belongs to the TGF-beta family. TGF beta-1 is found in high amounts in bone, especially in articular cartilage and chondrocytes. It's also higher in osteoarthritis (OA). TGF beta-1 controls cell growth, proliferation, differentiation, as well as apoptosis in a variety of cells. The precursor is cleaved into a latency-associated peptide (LAP) and a fully processed TGF beta-1 protein. TGF-beta may also interact with other TGF beta family members, forming heterodimers. TGF beta-1 is upregulated in tumor cells, according to studies. Camurati-Engelmann disease is a genetic condition that results from mutations in the TGF beta-1 gene. Human TGFB1/TGF-beta 1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human TGFB1/TGF-beta 1 Recombinant Protein (RPES1385)
Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/ml
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
12.8 kDa
AP Mol Mass:
13 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 50mM Glycine; 50mM NaCl; pH4.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
