Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The Indian Hedgehog (IHH) and Desert Hedgehog (DHH) are the other two members of the family. The hedgehog proteins are critical signaling molecules in embryonic development. SHH is expressed in many embryonic tissues and has important functions in patterning numerous systems including limbs and the brain. SHH also plays a key function in adulthood; it is involved in the division of adult stem cells, as well as the development of some cancer and other maladies. Human SHH is expressed as a 45kDa precursor; and undergoes a series of processing during secretion. After the removal of the signal peptide; a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface; which releases the repression of the activity of Smoothened (Smo); a G-protein coupled receptor; by Ptc. Human Sonic Hedgehog/SHH Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Sonic Hedgehog/SHH Recombinant Protein (RPES4771)
Product Code:
RPES4771
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Sonic Hedgehog Protein, SHH, HHG-1, SHH
Accession:
Q15465
Sequence:
Cys24-Gly197
Activity:
Immobilized Human SHH at 1µg/ml(100 ?l/well) can bind Human BOC-His(Cat: PKSH032123).
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
19.69 kDa
AP Mol Mass:
19 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 100mM NaCl; 1mM DTT; pH 7.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
