Suppressor of cytokine signaling 3, also known as SOCS-3, Cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3, SOCS3 and CIS3, is a protein which is widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. SOCS3 / CIS3 contains one SH2 domain and one SOCS box domain. SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 / CIS3 is involved in negative regulation of cytokines that signal through the JAK / STAT pathway. SOCS3 / CIS3 inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. SOCS3 / CIS3 suppresses fetal liver erythropoiesis. It regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. SOCS3 / CIS3 regulates IL-6 signaling. SOCS3 / CIS3 interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. SOCS3 / CIS3 could be used as a possible therapeutic agent for treating rheumatoid arthritis.
Product Name:
Human SOCS3/CIS3 Recombinant Protein (RPES1992)
Product Code:
RPES1992
Size:
50 µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
ATOD4,CIS3,Cish3,SOCS-3,SSI-3,SSI3
Accession:
O14543
Sequence:
Met 1-Leu 225
Fusion tag:
N-His & Trx
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human SOCS3 (O14543) (Met 1-Leu 225) was fused with a Trx and a polyhistide tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
41.9 kDa
AP Mol Mass:
46 kDa
Formulation:
Lyophilized from sterile 50mM Tris, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
