Signal-regulatory protein gamma (SIRPG/SIRP gamma) also known as CD172 antigen-like family member B, CD172g, and CD172g antigen, is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPG/SIRP gamma/CD172g is probable immunoglobulin-like cell surface receptor. On binding with CD47, SIRPG can mediate cell-cell adhesion. SIRPG/SIRP gamma is engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. SIRPG/SIRP gamma/CD172g is detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. This cytokine is mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, SIRPG is primarily expressed in the medulla on mature T-lymphocytes that have undergone thymic selection.
Product Name:
Human SIRP gamma/CD172g Recombinant Protein (RPES0679)
Product Code:
RPES0679
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Signal-Regulatory Protein Gamma, SIRP-Gamma, CD172 Antigen-Like Family Member B, Signal-Fegulatory Protein Beta-2, SIRP-b2, SIRP-Beta-2, CD172g, SIRPG, SIRPB2
Accession:
NP_061026.2
Sequence:
Met 1-Ser 364
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.Immobilized human SIRPG-His at 10 µg/ml (100 ?l/well) can bind human CD47-Fc , The EC50 of human CD47-Fc is 0.58-1.34 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human SIRPG (NP_061026.2) extracellular domain (Met 1-Ser 364) was expressed, with a polyhistidine tag at the C-terminus.
Purity:
> 96 % as determined by reducing SDS-PAGE.
Mol Mass:
34.7 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
