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Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-FC-Avi) Biotinylated (RPES6241)

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SKU:
RPES6241
Product Type:
Recombinant Protein
Species:
Human
$551

Description

Product Name:Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-FC-Avi) Biotinylated
Product Code:RPES6241
Size:20µg
Species:Human
Expression Host:HEK293 Cells
Synonyms:Sialic acid-binding Ig-like lectin 15, Siglec-15, CD33 antigen-like 3, CD33L3
Mol Mass:55.3 kDa
AP Mol Mass:58-75 kDa
Tag:C-Fc-Avi
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin Level:< 1.0 EU per μg of the protein as determined by the LAL method.
Bio Activity:Immobilized Human Siglec-15-Fc-Avi at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 13.7 ng/ml.
Sequence:Phe20-Thr263
Accession:Q6ZMC9
Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer informat
Reconstitution:Please refer to the printed manual for detailed information.
Background:Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

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