SH2 domain-containing protein 1A (SH2D1A / SAP) is a 128 amino acid protein, containing a single Src homology 2 (SH2) domain, flanked by 5 amino acids at the N-terminus and 25 amino acids at the C-terminus. The absence of a catalytic domain and the presence of an SH2 domain suggest that SH2D1A regulates one or more signal transduction pathways. SH2D1A interacts with signaling lymphocytic activation molecule (SLAM), which is a transmembrane protein expressed on the surface of activated T and B cells. SH2D1A (SAP) interacts via its SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of the cell-surface receptors, including CD150 / SLAM, CD84, CD229 / Ly-9, and CD244 / 2B4. SH2D1A was expressed in EBV-carrying, tumor phenotype representative (type I), but not in EBV-carrying lymphoblastoid cell line (LCL)-like (type III) or EBV-negative Burkitt lymphoma (BL) lines. It has been supposed to be related to the X-linked lymphoproliferative disease which is also known as Duncan's disease or Purtilo syndrome.
Product Name:
Human SH2D1A/SAP Recombinant Protein (RPES3275)
Product Code:
RPES3275
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
SH2 Domain-Containing Protein 1A, Duncan Disease SH2-Protein, Signaling Lymphocytic Activation Molecule-Associated Protein, SLAM-Associated Protein, T-Cell Signal Transduction Molecule SAP, SH2D1A,DSHP,EBVS,IMD5,LYP,MTCP1,SAP/SH2D1A,XLP,XLPD,XLPD1
Accession:
NP_002342.1
Sequence:
Pro 2-Lys 97
Fusion tag:
N-His
Activity:
1. Measured by its ability to bind recombinant human SLAMF1 in a functional ELISA.2. Measured by its ability to bind recombinant human SLAMF6 in a functional ELISA.3. Measured by its ability to bind recombinant mouse SLAMF6 in a functional ELISA.4. Measured by its ability to bind recombinant mouse CD84 in a functional ELISA.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human SH2D1A isoform 1 (NP_002342.1) (Pro 2-Lys 97) was expressed, with a polyhistidine tag at the N-terminus.
Purity:
> 94 % as determined by reducing SDS-PAGE.
Mol Mass:
15.6 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.5, 20% glycerol
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
