PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are molecules that have been observed to be signaling molecules that control a variety of cellular functions, such as cell growth, differentiation, mitosis, and oncogenic transformation. PTPN12 has a C-terminal PEST motif, which is involved in protein–protein interactions and may be linked to intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was found to bind and dephosphorylate a variety of cytoskeleton and cell adhesion molecules, including p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggested that it has regulatory functions in controlling cell shape and mobility. Human PTPN12 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human PTPN12 Recombinant Protein (RPES1106)
Product Code:
RPES1106
Size:
20µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
PTP-PEST,PTPG1
Accession:
AAA36529.1
Sequence:
Met 1-Gln355
Fusion tag:
Activity:
Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site .The specific activity is >20 ¦Ìmol/min/mg
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
