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Recombinant Human Proliferating cell nuclear antigen/PCNA Protein (RPCB1784)

SKU:
RPCB1784
Size:
10µg
Tag:
N-HA
Reactivity:
Human
Expression Host:
HEK293 cells
€139
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Description

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Product Name:Recombinant Human Proliferating cell nuclear antigen/PCNA Protein
SKU:RPCB1784
Size:10µg
Tag:N-HA
Reactivity:Human
Expression Host:HEK293 cells
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Human Proliferating cell nuclear antigen/PCNA Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:<0.1EU/μg
Purity:> 92% by SDS-PAGE.
Formulation:Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:5111

Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:P12004
Product ImageRecombinant Human Proliferating cell nuclear antigen/PCNA Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35 kDa.

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