Platelet-derived growth factor subunit A (PDGFA) belongs to the PDGF/VEGF family of growth factors. PADGFA is a secreted protein that is stored in platelet alpha-granules and released by platelets during injury. PDGFA is a potent mitogen for many cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. It plays an essential role in the regulation of embryonic development; cell proliferation; cell migration; survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis; normal development of the gastrointestinal tract; normal development of Leydig cells and spermatogenesis; normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. PDGF-B plays a key part in wound healing; the formation of heterodimers with PDGFB controls signal transmission. Human PDGF-AA Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20-160 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Platelet-derived growth factor AA is produced by our E.coli expression system and the target gene encoding Ser87-Thr211 is expressed.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
14.1 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 4mM HCl.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
