Oligodendrocyte-myelin glycoprotein; also known as OMG and OMGP; is a cell membrane protein which contains eight LRR (leucine-rich) repeats. OMG / OMGP is a glycosylphosphatidylinositol-anchored protein expressed by neurons and oligodendrocytes in the central nervous system (CNS). OMG / OMGP is a cell adhesion molecule contributing to the interactive process required for myelination in the central nervous system. OMG / OMGP play roles in both the developing and adult central nervous system. OMG / OMGP participats in growth cone collapse and inhibition of neurite outgrowth through its interaction with NgR; the receptor for Nogo. This function requires its leucine-rich repeat domain; a highly conserved region in OMgp during mammal evolution. OMG / OMGP leucine-rich repeat domain is also implicated in the inhibition of cell proliferation. OMG / OMGP may also be involved in the formation and maintenance of myelin sheaths. Cell proliferation; neuronal sprouting and myelination are crucial processes involved in brain development and regeneration after injury.
Product Name:
Human OMGP/OMG Recombinant Protein (RPES4842)
Product Code:
RPES4842
Size:
20µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
OMGP
Accession:
P23515-1
Sequence:
Met 1-Pro 416
Fusion tag:
C-His
Activity:
Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. Immobilized OMG (0.8 µg/ml; 100 ?l/well) will mediate >15% C6 cell adhesion.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human OMGP (P23515-1) (Met 1-Pro 416) without the pro peptide was expressed; fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
46 kDa
AP Mol Mass:
120-130 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
