Neurexin-3-beta; also known as Neurexin III-beta and NRXN3; is a single-pass type I membrane protein which belongs to the neurexin family. It contains one laminin G-like domain. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. Neurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors. They are encoded by several unlinked genes of which two; NRXN1 and NRXN3; are among the largest known human genes. Three of the genes ( NRXN1; NRXN2; NRXN3 ) utilize two alternate promoters and include numerous alternatively spliced exons to generate thousands of distinct mRNA transcripts and protein isoforms. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms; a much smaller number of transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. The alpha-neurexins contain EGF-like sequences and laminin G domains; and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. NRXN3 have been linked to genetic predisposition towards a number of conditions such as alcohol or drug addiction; or obesity.
Product Name:
Human NRXN3 Recombinant Protein (RPES4957)
Product Code:
RPES4957
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
C14orf60
Accession:
NP_620426.2
Sequence:
Met 1-Thr 357
Fusion tag:
C-His
Activity:
Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to NRXN3 coated plates (0.8 µg/ml and 100 ?l/well); approximately 30%-50% will adhere specifically after 60 minutes at 37 ?.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human NRXN3 beta isoform 2 (NP_620426.2) extracellular domain (Met 1-Thr 357) was expressed; with a polyhistidine tag at the C-terminus.
Purity:
> 94 % as determined by reducing SDS-PAGE.
Mol Mass:
36 kDa
AP Mol Mass:
50-60 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
