Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). N-terminal acidic region, a central basic heparin-binding segment, and a C-terminal cysteine knot structure are all present in Mature Human Noggin. Among vertebrates, Noggin is well conserved; thus, mature human Noggin has 99%; 99%; 98%; and 89% aa sequence identity with mouse; rat bovine; equine and chicken Noggin, respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds BMPs with distinct affinities. Some, such as BMP4, are highly bound, whereas others, such as BMP7, are less so. It antagonizes BMP bioactivities by binding to epitopes on BMPs required for both type I and type II receptors binding. Noggin is found in specific regions of the adult central nervous system and peripheral tissues including lung, skeletal muscle, and skin. Under certain conditions, culture of human embryonic stem cells (hESC) or neural stem cells may be induced; Noggin can be used to block BMP activity in order for stem cells to proliferate while keeping their unspecialized state. Alternatively, dopaminergic neurons may differentiate from stem cells. Human Noggin/NOG Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Noggin/NOG Recombinant Protein (RPES0723)
Product Code:
RPES0723
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Noggin, NOG
Accession:
Q13253
Sequence:
Gln28-Cys232
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
23 kDa
AP Mol Mass:
28-30 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 500mM NaCl; 2mM EDTA; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
