Recombinant Human Neddylin/NEDD8 Protein (RPCB0152)
- SKU:
- RPCB0152
- Size:
- 10µg
- Tag:
- C-His
- Reactivity:
- Human
- Expression Host:
- E. coli
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Description
| Product Name: | Recombinant Human Neddylin/NEDD8 Protein |
| SKU: | RPCB0152 |
| Size: | 10µg |
| Tag: | C-His |
| Reactivity: | Human |
| Expression Host: | E. coli |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Neddylin/NEDD8 Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | Please contact us for more information. |
| Purity: | > 97% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. |
| Gene ID: | 4738 |
Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits . Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes . Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates. NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies .
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | Q15843 |
 | Recombinant Human NEDD8 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 9 kDa. |
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