Pyruvate dehydrogenase kinase, isozyme 1, also known as [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial and PDK1, is a member of the PDK / BCKDK protein kinase family. PDK-1 is expressed predominantly in the heart. It contains one histidine kinase domain. Pyruvate dehydrogenase kinase (PDK) isoforms are molecular switches that downregulate the pyruvate dehydrogenase complex (PDC) by reversible phosphorylation in mitochondria. An inhibitory effect of lipoic acid on PDKs would result in less phosphorylation of E1 and hence increased PDC activity. At least two isoenzymic forms of pyruvate dehydrogenase kinase ( PDK-1 and PDK-2 ) may be involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase complex by phosphorylation. PDK-3 appears to have the highest specific activity among the three isoenzymes. PDK-1 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Product Name:
Human N6AMT1/HEMK2 Recombinant Protein (RPES2901)
Product Code:
RPES2901
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
C21orf127,HEMK2,m.HsaHemK2P,MTQ2,N6AMT,PRED28
Accession:
AAH11554.1
Sequence:
Met 1-Ser 186
Fusion tag:
C-His
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the mature form of human N6AMT1 (AAH11554.1) (Met 1-Ser 186) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
21.2 kDa
AP Mol Mass:
23 kDa
Formulation:
Lyophilized from sterile 20mM Tris, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
