Fibronectin type III domain-containing protein 5; the precursor of irisin; is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD); a 21 aa transmembrane domain; and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human; mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor ? coactivator 1? (PGC1?) and uncoupling protein1(UCP1); mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.
Product Name:
Human/Mouse/Rat Irisin/FNDC5 Recombinant Protein (RPES3660)
Product Code:
RPES3660
Size:
10µg
Species:
Human/Mouse/Rat
Expressed Host:
Human Cells
Synonyms:
Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin, FNDC5
Accession:
Q8NAU1
Sequence:
Asp32-Glu143
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human/Mouse/Rat FNDC5 is produced by our Mammalian expression system and the target gene encoding Asp32-Glu143 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
13.6 kDa
AP Mol Mass:
20-28 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
