Metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and participate in a variety of physiological functions, including morphogenesis, differentiation, angiogenesis, tissue remodeling, as well as pathological processes such as inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. Neutrophil collagenase (also known as Matrix metalloproteinase-8, MMP-8, and CLG1) is a member of the peptidase M10A. MMP-8 may affect the metastatic behaviour of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 in the tumour might help prevent lymph node metastasis. MMP-8 may aid with the metastatic potential of breast cancer cells via protection against lymph node metastasis, suggesting the need for anti-targets in drug discovery. MMP-8 helps with the resolution of inflammation and allows for the development of new therapies for wound healing problems. Human MMP8/CLG1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076) . The specific activity is > 250 pmoles/min/µg .(Activation description: The proenzyme needs to be activated by APMA for an activated form)
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the pro form of human MMP8 (NP_002415.1) (Phe 21-Gly 467) was expressed and purified, with an initial Met at the N-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
52 kDa
AP Mol Mass:
65 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
