Metalloproteinases (MMPs) are a group of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM). They play key roles in morphogenesis, differentiation, angiogenesis, and tissue remodeling as well as pathologic processes such as inflammation, arthritis, cardiovascular diseases, pulmonary ailments and tumour invasion. Macrophage metalloelastase, often known as Matrix metalloproteinase-12, Macrophage elastase, MMP12, and MMP-12, is a secreted enzyme from the Peptidase M10A family. MMP12 is a macrophage-secreted elastase that is highly activated in the liver and lung during infection with S. mansoni eggs and contains four hemopexin-like domains. MMP12 is a proteolytic enzyme that cleaves plasminogen to angiotensin, which has an anti-inflammatory affect. It has great elastolytic activity, which may be linked to tissue damage and remodeling. It may be linked to the prognosis of breast cancer patients. MMP12 downregulates the expression of specific ECM-degrading MMPs by limiting their activity. Like MMP12, MMP13 expression is highly dependent on IL-13 and type I I-IL-4 receptor signaling. MMP12 is a potent proinflammatory and oncogenic molecule. Inflammation and MMP12 up-regulation promote emphysema to lung cancer progression by facilitating the process. MMP12/MMP2/HME Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
MMP12/MMP2/HME Recombinant Protein (RPES4883)
Product Code:
RPES4883
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
HME,ME,MME,MMP-12
Accession:
NP_002417.2
Sequence:
Gly106-Asn268
Fusion tag:
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 800 pmoles/min/¦Œg
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding human MMP12 (NP_002417.2) (Gly106-Asn268) was expressed with a N-terminal Met.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
18.2 kDa
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from sterile 10 mM Hepes, 2 mM CaCl2, 250 mM NaCl, pH 7.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
