Matrix metallopeptidase 3 (abbreviated as MMP3) is also known as stromelysin 1 and progelatinase. MMP3 is a matrix metalloproteinase (MMP) member that plays a role in the breakdown of extracellular matrix during normal physiological functions such as embryonic development, reproduction, tissue regeneration, and disease processes including arthritis and metastasis. MMP3 acts in the extracellular matrix as a secreted zinc-dependent endopeptidase. Alpha2-macroglobulin and tissue metalloproteinase inhibitors (TIMPs) are two major endogenous inducers that activate this protein. Collagen types II, III, IV, IX, and X are all targeted by MMP3. It also targets proteoglycans, fibronectin, laminin, and elastin. MMP3 may also activate other MMPs, including MMP1, MMP7, and MMP9, which is why it's so important in connective tissue remodeling. MMPs are implicated in a variety of diseases, including arthritis, chronic ulcers, encephalomyelitis, and cancer. Synthetic or natural MMPs inhibitors decrease metastasis, whereas up-regulation of MMPs promotes cancer cell invasion. Human MMP-3 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nva-WR-K(Dnp)-NH2, AnaSpec, Catalog # 27114.The specific activity is >300 pmoles/min/µg.(Activation description: The proenzyme needs to be activated by Chymotrypsin for an activated form)
Endotoxin:
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Protein Construction:
A DNA sequence encoding the human MMP3 (AAA36321.1) N-terminal fragment (Tyr 18-Thr 272) was expressed and purified.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
