Recombinant Human Leukemia inhibitory factor/LIF Protein (RPCB1058)
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- SKU:
- RPCB1058
- Size:
- 10µg
- Tag:
- NO-tag
- Reactivity:
- Human
- Expression Host:
- HEK293 cells
Description
| Product Name: | Recombinant Human Leukemia inhibitory factor/LIF Protein |
| SKU: | RPCB1058 |
| Size: | 10µg |
| Tag: | NO-tag |
| Reactivity: | Human |
| Expression Host: | HEK293 cells |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Leukemia inhibitory factor/LIF Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | <0.1EU/μg |
| Purity: | > 92% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 3976 |
Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | P15018 |
 | Recombinant Human Leukemia inhibitory factor/LIF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-45 kDa. |
 | Recombinant Human LIF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.14-0.54 ng/mL, corresponding to a specific activity of 1.85×106-7.14×106 units/mg. |
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