Leptin is a hormone produced by white fat cells that has an impact on food consumption and energy balance. Leptin works via signals that travel through the hypothalamus OB-R pathway. Leptin's impact on reducing body weight and controlling blood glucose level has been shown in animal studies. Mice without leptin deficiency became massively obese and had a higher chance of diabetes when leptin-related mutations were introduced in the obese gene. Metabolic rate is reduced in leptin deficiency. Leptin-deficient mice are less active and have a reduced body temperature than normal mice. The human protein is nearly identical to the mouse protein, sharing about 84 percent sequence identity. The mature protein sequence of human leptin has an amino acid length of 167 residues, including a 21-amino-acid signal sequence and a 146-amino-acid core protein. Human Leptin Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Immobilized Human Leptin at 10µg/ml(100 ?l/well) can bind Human CD295-His(Cat: PKSH033606).
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
16.1 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
