Langerin (CD207) is a type II transmembrane glycoprotein which is member K of the C-type lectin domain family. Langerin is used as a marker for Langerhans cells (LCs) which represent the immature dendritic cells in the epidermis. Langerin is necessary and sufficient for Birbeck granule formation. Human langerin sequence contains a 43 aa cytoplasmic domain, a 21 aa transmembrane domain and a 264 aa extracellular domain (ECD) that contains a coiled-coil domain and a single C-type lectin domain. Human langerin shares 68%, 62%, 71% aa identity with mouse, rat and bovine langerin ECD, respectively. Trimerization greatly increases the lectin binding affinity. Langerin internalizes endogenous proteins such as type I procollagen. Internalization by LC is thought to lead to suppression of self reactions. Langerin also mediates endocytosis of non-peptide antigens containing mannose, N-acetyl glucosamine and fucose that are expressed by mycobacteria and fungae.
Product Name:
Human Langerin/CD207 Recombinant Protein (RPES3292)
Product Code:
RPES3292
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
CD207 antigen, langerin, CD207, C-type lectin domain family 4 member K, C-type lectin domainfamily 4, member K
Accession:
Q9UJ71
Sequence:
Tyr64-Pro328(Val278Ala)
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Langerin is produced by our Mammalian expression system and the target gene encoding Tyr64-Pro328(Val278Ala) is expressed with a 6His tag at the N-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
31.5 kDa
AP Mol Mass:
30-40 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
