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Recombinant Human Klotho beta/KLB Protein (RPCB0090)

SKU:
RPCB0090
Size:
10µg
Tag:
C-His
Reactivity:
human
Expression Host:
HEK293 cells
$167
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Description

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Product Name:Recombinant Human Klotho beta/KLB Protein
SKU:RPCB0090
Size:10µg
Tag:C-His
Reactivity:human
Expression Host:HEK293 cells
Protein Description:High quality, high purity and low endotoxin recombinant Recombinant Human Klotho beta/KLB Protein , tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:< 1 EU/μg of the protein by LAL method.
Purity:> 95% by SDS-PAGE.
Formulation:Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Gene ID:152831

Klotho beta/KLB iis a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue . Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex . The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) . Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake . Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes . However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible .

Storage:Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution:Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Swiss-Prot:Q86Z14
Product ImageRecombinant Human Klotho beta/KLB Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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