Kelch-like ECH-associated protein 1 (cytosolic inhibitor of Nrf2/Kelch-like protein 19/KEAP1/INRF2) is a cytoplasm and nucleus protein which contains one BACK (BTB/Kelch associated) domain; one BTB (POZ) domain and six Kelch repeats. KEAP1 / INRF2 is broadly expressed; with highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor; which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 binds to and targets NRF2 for ubiquitination and proteolysis, thus leading to the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. The mechanism of action is through ubiquitination and degradation by the proteasome of NFE2L2/NRF2, resulting in the inhibition of its transcriptional activity and antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It is a ubiquitination and degradation target for PGAM5 by the proteasome. Human KEAP1/INRF2 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human KEAP1/INRF2 Recombinant Protein (RPES4295)
Product Code:
RPES4295
Size:
50µg
Species:
Human
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
INRF2,KEAP-1,KLHL19
Accession:
Q14145
Sequence:
Gln2-Cys624
Fusion tag:
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human KEAP1 (Q14145) (Gln2-Cys624) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
