Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 amino acid precursor protein containing a 25 amino acid signal peptide and a 152 amino acid mature protein. Human and mouse IL7 share 65% sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT; which regulate lymphocyte survival; glucose uptake; proliferation; and differentiation. IL-7 is also associated with cytoplasmic IL2-R gamma for signal transduction.
Product Name:
Human Interleukin-7/IL-7 Recombinant Protein (RPES2582)
Product Code:
RPES2582
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Interleukin-7, IL-7, IL7
Accession:
P13232
Sequence:
Asp26-His177
Fusion tag:
C-6His
Activity:
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 50-300 pg/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Asp26-His177 is expressed with a 6His tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
18.4 kDa
AP Mol Mass:
25 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
